產(chǎn)品編號 | bsm-33318M-Cy5 |
英文名稱 | Mouse Anti-Firefly Luciferase/Cy5 Conjugated antibody |
中文名稱 | Cy5標記的熒光素酶單克隆抗體 |
別 名 | ec 1.13.12.7; Firefly; Firefly Luciferase Luciferase; Luciferin 4 monooxygenase. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
產(chǎn)品類型 | 標簽抗體 |
研究領(lǐng)域 | 激酶和磷酸酶 昆蟲 |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號 | 11D6 |
交叉反應(yīng) | Recombinant protein |
產(chǎn)品應(yīng)用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 61kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Firefly Luciferase |
亞 型 | IgG1 |
純化方法 | affinity purified by Protein G |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. Function: Produces green light with a wavelength of 562 nm. Subcellular Location: Peroxisome. Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Database links: SwissProt: P08659 Firefly (Photinus pyralis) Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復(fù)方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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