| 產(chǎn)品編號 | bs-18936R-BF488 |
| 英文名稱 | Rabbit Anti-MICALL2/JRAB/BF488 Conjugated antibody |
| 中文名稱 | BF488標(biāo)記的MICAL樣蛋白2抗體 |
| 別 名 | FLJ23471; FLJ41996; FLJ44858; FLJ45410; JRAB; Junctional Rab13 binding protein; MGC46023; MICAL L2; MICAL like 2; MICAL like protein 2; MICALL2. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 鋅指蛋白 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat, (predicted: Human, Mouse, ) |
| 產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 55kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MICALL2/JRAB |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: JRAB is a 904 amino acid protein with one CH (calponin-homology) domain and one LIM zinc-binding domain. JRAB has been shown to interact with Rab13 and Rab8 to facilitate cellular transport of claudin-1, occludin, and E-cadherin. This interaction is vital for the coordination of the assembly of tight junctions (TJs) and adherens junctions (AJs). Dynamic turnover (endocytic recycling) of cell-to-cell AJs and TJs is essential for epithelial morphogenesis during normal development and differentiation. The endocytic recycling of occludin and cluadin proteins is part of an ongoing process of restructuring and maintaining cell junctions, especially at TJs. JRAb and Rab13 have also been implicated in the carcinoma metastasis event of epithelial cell scattering. This event shows Rab13 and JRAB colocalizing with F-Actin in lamellipodial structures prior to cell scattering. Function: Effector of small Rab GTPases which is involved in junctional complexes assembly through the regulation of cell adhesion molecules transport to the plasma membrane and actin cytoskeleton reorganization. Regulates the endocytic recycling of occludins, claudins and E-cadherin to the plasma membrane and may thereby regulate the establishment of tight junctions and adherens junctions. In parallel, may regulate actin cytoskeleton reorganization directly through interaction with F-actin or indirectly through actinins and filamins. Most probably involved in the processes of epithelial cell differentiation, cell spreading and neurite outgrowth. Subunit: Interacts with RAB13 (GTP-bound form); competes with RAB8A and is involved in tight junctions assembly. Interacts with RAB8A; competes with RAB13 and is involved in E-cadherin endocytic recycling By similarity. Interacts with RAB8B By similarity. Interacts (preferentially in opened conformation) with ACTN1 and ACTN4; stimulated by RAB13 activation By similarity. Interacts (via CH domain) with the filamins FLNA, FLNB and FLNC (via actin-binding domain). Subcellular Location: Cell membrane; Cytoplasmic; cytoskeleton Similarity: Contains 1 CH (calponin-homology) domain. Contains 1 LIM zinc-binding domain. Database links: Entrez Gene: 79778 Human SwissProt: Q8IY33 Human Unigene: 376617 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
