產(chǎn)品編號 | bs-14518R-BF594 |
英文名稱 | Rabbit Anti-EFHA2/BF594 Conjugated antibody |
中文名稱 | BF594標記的EFHA2蛋白抗體 |
別 名 | EF-HA2; 2900075B16Rik; DKFZp313A0139; EF hand domain family A2; EF hand domain family member A2; EF-hand domain-containing family member A2; Efha2; MICU3_HUMAN. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 細胞生物 信號轉(zhuǎn)導 結合蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | |
產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 61kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human EFHA2 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: The EF-hand domain is a twelve amino acid loop motif that is commonly found in proteins that participate in calcium-binding events within the cell. EF-hand domains generally exist in a pair that, together, form a stable four-helix bundle that enables the binding of calcium ions. EF-HB (EF-hand domain-containing family member B) is a 831 amino acid protein that contains two EF-hand domains, suggesting a role in calcium-mediated events throughout the cell. There are three isoforms of EF-HB that are produced as a result of alternative splicing events. The gene encoding EF-HB is located on human chromosome three,which is made up of about 214 million bases encoding over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Other well-studied calcium binding proteins that contain EF-hand motifs include calmodulin (CaM), Troponin C, myosin regulatory light chain (MYL) and S-100 proteins Function: May play a role in mitochondrial calcium uptake (By similarity). Subcellular Location: Mitochondrion (By similarity). Membrane; Single-pass membrane protein (Potential). Similarity: Contains 2 EF-hand domains. Database links: Entrez Gene: 151651 Human SwissProt: Q8N7U6 Human Unigene: 120196 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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