| 產品編號 | bs-4504R-HRP |
| 英文名稱 | Rabbit Anti-PRRSV-GP5/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的豬藍耳病病毒GP5蛋白抗體 |
| 別 名 | Porcine reproductive and respiratory syndrome, PRRS; PRRSVgp6 GP5 glycosylated envelope protein [ Porcine reproductive and respiratory syndrome virus ]; PRRSV GP5. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 免疫學 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | |
| 產品應用 | WB=1:500-2000 ELISA=1:100-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 22kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from PRRSV GP5 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: PRRSV is a small, enveloped RNA virus. It contains a single-stranded, positive-sense, RNA genome with a size of approximately 15 kilobases. The genome contains nine open reading frames. PRRSV is a member of the genus Arterivirus, family Arteriviridae, order Nidovirales. Subclinical infections are common, with clinical signs occurring sporadically in a herd. Clinical signs include reproductive failure in sows such as abortions and giving birth to stillborn or mummified fetuses, and cyanosis of the ear and vulva. In neonatal pigs, the disease causes respiratory distress, with increased susceptibility to respiratory infections such as Glasser's disease. Function: The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products. Nsp1 is essential for viral subgenomic mRNA synthesis. Nsp1-alpha inhibits IFN-beta production. Counteracts the action of NF-kappaB by decreasing the phosphorylation of IkappaB-alpha, such that the degradation of IkappaB-alpha is suppressed. This leads to the blockage of NF-kappaB nuclear translocation and thus interference of NF-kappaB activation. Also seems to inhibit IRF3-dependent pathways. Nsp1-beta seems to inhibit the translocation of STAT1 into the cell nucleus. Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity. Deubiquitinates host NFKBIA, thereby interfering with NFKBIA degradation and impairing subsequent NF-kappa-B activation. The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein. The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Post-translational modifications: Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. Nsp1 is autocleaved into two subunits, Nsp1-alpha and Nsp1-beta. There are two alternative pathways for processing. Either nsp4-5 is cleaved, which represents the major pathway or the nsp5-6 and nsp6-7 are processed, which represents the minor pathway. The major pathway occurs when nsp2 acts as cofactor for nsp4. Similarity: Belongs to the arteriviridae polyprotein family. Contains 1 (+)RNA virus helicase ATP-binding domain. Contains 1 (+)RNA virus helicase C-terminal domain. Contains 1 AV MBD (arterivirus metal-binding) domain. Contains 1 peptidase C31 domain. Contains 1 peptidase C32 domain. Contains 1 peptidase C33 domain. Contains 1 peptidase S32 domain. Contains 1 RdRp catalytic domain. Database links: Entrez Gene: 1494885 PRRSR SwissProt: Q9WJB2 PRRSR Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 豬繁殖與呼吸綜合征(Porcine reproductive and respiratory syndrome，PRRS)也稱豬藍耳病，是由動脈炎病毒科動脈炎病毒屬的豬繁殖與呼吸綜合征病毒,(Porcine reproductive and respiratory syndrome virus，PRRSV)引起的一種嚴重的病毒性傳染病，該病以引起母豬的繁殖障礙、仔豬和育成豬呼吸道癥狀及高死亡率為主要特征。自1987年首次發(fā)現(xiàn)以來，該病廣泛流行于世界各主要養(yǎng)豬國家和地區(qū)，已成為威脅養(yǎng)豬業(yè)的主要疫病之一。2006年以來，由該病毒變異株引起的高致病性豬藍耳病(Highlypathogenicporcinereproductiveandrespiratorysyndrome，HPPRRS)在我國廣泛流行給養(yǎng)豬業(yè)帶來了巨大的損失。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
