| 產(chǎn)品編號 | bs-9315R-BF594 |
| 英文名稱 | Rabbit Anti-MKLP1/BF594 Conjugated antibody |
| 中文名稱 | BF594標記的有絲分裂驅(qū)動蛋白樣1抗體 |
| 別 名 | CHO 1; CHO1; KIF 23; KIF23; KIF23_HUMAN; Kinesin family member 23; Kinesin like 5; Kinesin like protein 5; Kinesin like protein KIF 23; Kinesin like protein KIF23; Kinesin-like protein 5; Kinesin-like protein KIF23; KNS L5; KNSL 5; KNSL5; Mitotic kinesin like 1; Mitotic kinesin like protein 1; Mitotic kinesin-like protein 1; MKLP 1. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 心血管 細胞生物 細胞周期蛋白 細胞分化 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | |
| 產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 110kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MKLP1 (221-310aa) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in two transcript variants encoding two different isoforms. Function: Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Subunit: Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Interacts with RACGAP1; the interaction is direct. Interacts with ECT2 and PRC1. Interacts with ANXA11 during cytokinesis. Interacts with BIRC6/bruce and USP8/UBPY. Subcellular Location: Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Localizes to the interzone of mitotic spindles. Post-translational modifications: Ubiquitinated. Deubiquitinated by USP8/UBPY. Similarity: Belongs to the kinesin-like protein family. Contains 1 kinesin-motor domain. Database links: Entrez Gene: 9493 Human Entrez Gene: 71819 Mouse Omim: 605064 Human SwissProt: Q02241 Human Unigene: 270845 Human Unigene: 259374 Mouse Unigene: 63734 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
