| 產(chǎn)品編號 | bs-1132R-BF488 |
| 英文名稱 | Rabbit Anti-Somatostatin 28/BF488 Conjugated antibody |
| 中文名稱 | BF488標記的生長抑素抗體 |
| 別 名 | GRIH; Growth hormone release inhibiting factor; SMS_HUMAN; SMST; SST; Somatostatin 14; Somatostatin14; Somatostatin-14; somatostatin SS14; Somatostatin 28; Somatostatin28; Somatostatin-28; somatostatin SS28. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 神經(jīng)生物學 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, Rat, (predicted: Human, Chicken, Pig, Cow, Sheep, ) |
| 產(chǎn)品應用 | Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 13kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Somatostatin 28 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The hormone somatostatin has active 14 aa and 28 aa forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells. [provided by RefSeq, Jul 2008]. Function: Somatostatin inhibits the release of somatotropin. Subcellular Location: Secreted. Similarity: Belongs to the somatostatin family. Database links: Entrez Gene: 396279 Chicken Entrez Gene: 6750 Human Entrez Gene: 20604 Mouse Omim: 182450 Human SwissProt: P33094 Chicken SwissProt: P61278 Human SwissProt: P60041 Mouse Unigene: 12409 Human Unigene: 34418 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 生長抑素是胰島D細胞的一種多肽激素，主要用于胰島細胞的功能性分類和消化道粘膜中內(nèi)分泌細胞及腫瘤的研究。 生長抑素在體內(nèi)分布廣泛。在神經(jīng)系統(tǒng)中，廣泛存在于中樞和外周神經(jīng)系統(tǒng)，在腦內(nèi)以下丘腦正中隆起的濃度為最高。在新皮層，邊緣系統(tǒng)下杏仁核，海馬等部位也廣泛存在，以皮層含量最高。在脊髓后根和三叉神經(jīng)神經(jīng)節(jié)內(nèi)的一級神經(jīng)元中，亦含生長抑素的免疫反應性物質(zhì)。生長抑素廣泛存在于胃腸道粘膜的“D”細胞，以胃竇和胃體最高，在腸內(nèi)越往下含量越低?！癉”細胞有長的胞漿突起，在幽門腺區(qū)止于G細胞和“嗜鉻細胞”，在泌酸腺區(qū)止于壁細胞和其他上皮細胞。生長抑素通過旁分泌機制由突起釋放到G細胞和壁細胞膜上，抑制胃泌素和HCI分泌，在胰腺內(nèi)，生長抑素由胰島“D”細胞分泌，通過血液循環(huán)對胰島及消化道起作用，作為旁分泌調(diào)節(jié)胰島功能。 生長抑素的生理作用為：抑制垂體生長激素、促甲狀腺激素、促腎上腺皮質(zhì)激素和催乳類的釋放，也抑制各種胃腸激素的釋放，抑制胃泌素、促胰液素、膽囊收縮素、胃動素、胰多肽、胰高血糖素、腸高血糖素等的釋放。同時也抑制胃酸、胃蛋白酶、胰蛋白酶及唾液淀粉酶的分泌。 |
| 產(chǎn)品圖片 |
Blank control:Mouse brain .
Primary Antibody (green line): Rabbit Anti-Somatostatin 14 antibody (bs-1132R-BF488)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
