| 產(chǎn)品編號 | bs-7844R-HRP |
| 英文名稱 | Rabbit Anti-GSK3B interacting protein/Ninein/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的GSK3B相互作用蛋白抗體 |
| 別 名 | Centrosome Marker; Centrosome Marker; Glycogen synthase kinase 3 beta interacting protein; Centrosome Marker; Glycogen synthase kinase 3 beta interacting protein; GSK3B interacting protein; h Glycogen synthase kinase 3 beta-interacting protein; hNinein; KIAA1565; NIN; NIN protein; NIN_HUMAN; ninein (GSK3B interacting protein); Ninein; Ninein centrosomal protein; SCKL7. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 信號轉(zhuǎn)導 細胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 243kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Ninein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Ninein is a centrosomal protein required for the positioning and anchorage of the microtubule minus-end in epithelial cells. It may also be a centrosome maturation factor and may be involved in microtubule nucleation. Ninein is a component of the core centrosome and is arranged in a tubular shape with an open and a closed end. Function: Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. Subunit: Homooligomer. Interacts with GSK3B (GSK3-beta) via its C-terminus domain. Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3-beta. Subcellular Location: Cytoplasm, cytoskeleton, centrosome. Tissue Specificity: Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5. Post-translational modifications: Phosphorylated by Aurora A (AIK) and PKA kinases but not CK2 or Aurora B (AIM). Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 5 EF-hand domains. Database links: Entrez Gene: 51199 Human Entrez Gene: 18080 Mouse Omim: 608684 Human SwissProt: Q8N4C6 Human SwissProt: Q61043 Mouse Unigene: 310429 Human Unigene: 569387 Human Unigene: 244047 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
