| 產(chǎn)品編號 | bs-2397R-Bio |
| 英文名稱 | Rabbit Anti-Endothelial lipase/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的內(nèi)皮脂肪酶抗體 |
| 別 名 | LIPG; EC 3.1.1.3; EDL; EL; Endothelial cell-derived lipase; Endothelial lipase; Endothelial lipase precursor; Lipase endothelial; LIPE_HUMAN; LIPG; Lipoprotein lipase H; PL; PRO719. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 心血管 細胞生物 免疫學 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | 55kDa |
| 交叉反應(yīng) | Mouse, Rat, (predicted: Human, Chicken, Dog, Pig, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 55kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Endothelial lipase |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]. Function: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Subunit: Head to tail homodimer. Subcellular Location: Secreted. Tissue Specificity: High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocytes and osteosarcoma cell lines. Not detected in heart, brain and muscle. Similarity: Belongs to the AB hydrolase superfamily. Lipase family. Contains 1 PLAT domain. Database links: Entrez Gene: 9388 Human Omim: 603684 Human SwissProt: Q9Y5X9 Human Unigene: 465102 Human Unigene: 199051 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. EL被確定為甘油三酯脂肪酶家族的新成員，由于EL是由血管內(nèi)皮細胞合成和分泌，因此被命名為內(nèi)皮脂肪酶，相應(yīng)的基因命名為內(nèi)皮脂肪酶基因(endothelial lipase gene，LIPG)。EL也是一種分泌型蛋白，由483個氨基酸組成，分子量約55kD，在人體多種組織中都有表達：冠狀動脈、胎盤、甲狀腺、肝臟、肺臟、腎臟、睪丸和卵巢等組織中都可發(fā)現(xiàn)。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
