| 產(chǎn)品編號 | bs-0096R-Gold |
| 英文名稱 | Rabbit Anti-alpha 1 Antitrypsin/Gold Conjugated antibody |
| 中文名稱 | 膠體金標記的α-1抗胰蛋白酶抗體 |
| 別 名 | A1-Antitrypsin; Tryptase; Alpha-1-Antitrypsin; alpha 1-antitrypsin; A1A; A1AT; AAT; Alpha 1 antiproteinase; Alpha 1 protease inhibitor; alpha-1-AT; alpha1 PI; alpha1 proteinase inhibitor; Clade A (alpha 1 antiproteinase antitrypsin) member 1; MGC23330; MGC9222; PI; PI; PRO2275; Protease inhibitor 1 (anti elastase); Serpin A1; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 1; SerpinA1; A1AT_HUMAN; Alpha-1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul（10nm 15nm 35nm） |
| 研究領(lǐng)域 | 心血管 免疫學(xué) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Rat, (predicted: Mouse, Dog, Pig, Cow, ) |
| 產(chǎn)品應(yīng)用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human alpha 1 Antitrypsin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產(chǎn)品介紹 |
background: Alpha-1-antitrypsin is a protease inhibitor, deficiency of which is associated with emphysema and liver disease. The protein is encoded by a gene (PI) located on the distal long arm of chromosome 14. Function: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). Subcellular Location: Secreted. Short peptide from AAT: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Plasma. Post-translational modifications: N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. DISEASE: Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD) [MIM:613490]. A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Similarity: Belongs to the serpin family. Database links: Entrez Gene: 5265 Human Omim: 107400 Human SwissProt: P01009 Human SwissProt: P07758 Mouse Unigene: 525557 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. α1-抗胰蛋白酶（A1AT）缺乏是最常見的遺傳代謝病，能引起肺和肝的損傷。α1抗胰蛋白酶為呼吸系統(tǒng)的非特異性可溶因子，與呼吸道抵抗力關(guān)系密切，它可抑制多種酶的活性，包括細菌的酶，以及中性白細胞溶酶體分泌的蛋白酶、彈性蛋白酶、膠原酶、纖維蛋白溶酶和凝血酶。α1抗胰蛋白酶的缺乏與慢性阻塞性肺病的形成關(guān)系密切，因為它的缺乏，不能及時控制感染和炎癥產(chǎn)生的多種蛋白酶，而造成肺組織破壞。 α1-抗胰蛋白酶由肝細胞產(chǎn)生,是一種分子量為45-56kDa的糖蛋白,它能抑制蛋白酶、彈性蛋白酶、膠原酶等多種水解酶的活性。 AAT也是一種糖蛋白，主要用于遺傳性AAT缺乏癥和良性惡性肝腫瘤、內(nèi)胚竇瘤、組織細胞性淋巴瘤以及胰腺癌、胃癌、結(jié)腸癌等各種腫瘤的研究。此抗體與大、小鼠、狗和豬有交叉反應(yīng)。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
