| 產(chǎn)品編號 | bs-1807R-BF488 |
| 英文名稱 | Rabbit Anti-GPR78/BF488 Conjugated antibody |
| 中文名稱 | BF488標(biāo)記的G蛋白偶聯(lián)受體78抗體 |
| 別 名 | GPCR GPR78; G protein-coupled receptor 78; G protein coupled receptor GPR78; G-protein coupled receptor 78; GPR78; GPR78_HUMAN; PRO19818; UNQ5925. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 39kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR78 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. Function: Orphan receptor. Displays a significant level of constitutive activity. Its effect is mediated by G(s)-alpha protein that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: High level of expression in placenta. Expressed throughout the brain at low level. No expression detected in skeletal muscle, lung, heart, liver, pancreas, or kidney. Similarity: Belongs to the G-protein coupled receptor 1 family. Database links: Entrez Gene: 27201 Human Omim: 606921 Human SwissProt: Q96P69 Human Unigene: 350588 Human Unigene: 740355 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |
Blank control: 293T Cells(blue).
Primary Antibody: Rabbit Anti-GPR78/AF488 Conjugated antibody (bs-1807R-BF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-1807R-BF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
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