| 產(chǎn)品編號 | bsm-60899R |
| 英文名稱 | STAT5A Recombinant Rabbit mAb |
| 中文名稱 | 信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子5重組兔單抗 |
| 別 名 | signal transducers and activators of transduction 5; MGF; Signal Transducer and Activator of Transcription 5A; STAT 5; STAT 5A; STAT 5B; STAT5A; STAT5B; Transcription factor STAT5A; STA5B_HUMAN; STA5A_HUMAN. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 15H8 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 90 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human STAT5 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq, Jul 2008] Function: Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. Subunit: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1 and SOCS7. Interacts with ERBB4. Subcellular Location: Cytoplasm. Nucleus. Note=Mostly cytoplasmic during pregnancy, whereas it becomes predominantly nuclear during early lactation. It is translocated into the nucleus in response to phosphorylation. Tissue Specificity: Expressed in heart, lung, and weakly in muscle. Post-translational modifications: Tyrosine phosphorylated in response to IL2, IL3, IL7, IL15, KITLG/SCF, CSF2/GMCSF, GH1, PRL, EPO and THPO. Tyrosine phosphorylated in response to signaling via activated FLT3 and KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). Similarity: Belongs to the transcription factor STAT family. Contains 1 SH2 domain. SWISS: P42229 Gene ID: 6776 Database links: Entrez Gene: 6776 Human Entrez Gene: 20850 Mouse Omim: 601511 Human SwissProt: P42229 Human SwissProt: P42230 Mouse Unigene: 437058 Human Unigene: 277403 Mouse Unigene: 154399 Rat 轉(zhuǎn)錄調(diào)節(jié)因子（Transcriptin Regulators） STAT-信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子蛋白家族是近年來發(fā)現(xiàn)的一類轉(zhuǎn)錄因子.通過JAK-STAT途徑介導(dǎo)許多由細(xì)胞因子誘導(dǎo)的反應(yīng),參與細(xì)胞的信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in EDTA buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (STAT5A) Monoclonal Antibody, Unconjugated (bsm-60899R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (STAT5A) Monoclonal Antibody, Unconjugated (bsm-60899R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023)instructionsand DAB staining.
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