| 產(chǎn)品編號(hào) | bsm-60206R |
| 英文名稱 | β-Hydroxybutyryl-Histone H3 (Lys9) Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | E6A5 |
| 交叉反應(yīng) | Human,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 組蛋白能被多種酶催化發(fā)生修飾，比如乙?；谆?，磷酸化和泛素化等。賴氨酸β-羥基丁酰化（Kbhb）是近期發(fā)現(xiàn)的一種主要富集在活性基因啟動(dòng)子區(qū)的組蛋白標(biāo)記。 研究表明，在培養(yǎng)細(xì)胞時(shí)，提高β-羥基丁酸鹽濃度；長期禁食或用鏈脲佐菌素誘導(dǎo)小鼠糖尿病酮癥，可顯著提高細(xì)胞或小鼠肝臟中的Kbhb水平。 組蛋白Kbhb是一種可以將代謝與基因表達(dá)偶聯(lián)的全新表觀遺傳調(diào)控機(jī)制，為研究染色質(zhì)調(diào)控以及一些重要生理病理疾病比如糖尿病，癲癇和腫瘤中β-羥基丁酸鹽的多種功能提供了新途徑。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium 3-ohbutyrylate (50mM, 72hr)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Tissue: Rat colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60206R
|
