產(chǎn)品編號 | bsm-60430R |
英文名稱 | AMACR Recombinant Rabbit mAb |
中文名稱 | |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | H4A1 |
交叉反應(yīng) | Human |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | kDa |
檢測分子量 | |
細(xì)胞定位 | 細(xì)胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 | 催化α-甲基支鏈脂肪酰基-CoA酯的(R)-和(S)-立體異構(gòu)體的相互轉(zhuǎn)化。 僅作用于輔酶A硫酯,不作用于游離脂肪酸,并且接受多種α-甲基酰基-CoA作為底物,包括角鯊?fù)轷;?CoA,三羥基coprostanoyl-CoA(膽汁酸合成的中間體)和芳基丙酸(如抗壞血酸) -炎癥藥布洛芬(2-(4-異丁基苯基)丙酸),但既不是3-甲基支鏈的也不是線性鏈的?;o酶A。 |
產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: LNCAP, 2: Human liver
Protein loading quantity: 20 μg
Exposure time: 30s
Predicted MW: 42 kDa
Observed MW: 42 kDa
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AMACR Monoclonal Antibody, Unconjugated(bsm-60430R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AMACR Monoclonal Antibody, Unconjugated(bsm-60430R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AMACR Monoclonal Antibody, Unconjugated(bsm-60430R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AMACR Monoclonal Antibody, Unconjugated(bsm-60430R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AMACR Monoclonal Antibody, Unconjugated(bsm-60430R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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