| 產品編號 | bs-48021P |
| 英文名稱 | Recombinant human EPO protein (Active, HEK293) |
| 中文名稱 | 重組人促紅細胞生成素 (活性的, Tag free) |
| 別 名 | EPO_HUMAN; Erythropoietin; Epoetin; EP; DBAL; ECYT5; MVCD2; 人促紅細胞生成素; 人促紅細胞生成素重組蛋白; 重組人促紅細胞生成素; 人重組人促紅細胞生成素; 重組人EPO; |
| 理論分子量 | 36 kDa |
| 檢測分子量 | 36-40 |
| 性 狀 | Lyophilized |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | Human |
| 序 列 | 28-193/193 |
| 純 度 | >95% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內毒素 | <0.5 EU/μg as determined by LAL. |
| 表達系統(tǒng) | HEK293 cell |
| 活性 | Yes |
| 標簽 | Tag free |
| 緩 沖 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存條件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
Recombinant human erythropoietin(EPO) expressed in engineered human cells. EPO belongs to the EPO/TPO protein family. EPO is an acidic hormone mainly produced by kidneys. EPO promotes erythroid progenitor cell differentiation and initiates hemoglobin synthesis. EPO acts by binding to a specific erythropoietin receptor (EPOR) present on the red blood cell precursor in the bone marrow, stimulating these cells to transform into mature erythrocytes. The EPO sensitivity progressively increases with differentiation of immature progenitor cells. This recombinant human EPO is produced by human cells. (Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of human TF-1 cells (human erythroleukemic indicator cell line). EPO activity is 79KU/mg. Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| 產品圖片 |
The purity of the protein is greater than 95% as determined by non reduced and reduced SDS-PAGE
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
