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STAT5b Recombinant Rabbit mAb (bsm-52238R)  
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產(chǎn)品編號 bsm-52238R
英文名稱 STAT5b Recombinant Rabbit mAb
中文名稱 信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子5b重組兔單抗
別    名 Transcription factor STAT5B; Signal transducer and activator of transcription 5B; STA5B_HUMAN; STAT5.  
研究領(lǐng)域 腫瘤  染色質(zhì)和核信號  信號轉(zhuǎn)導(dǎo)  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 12A1
交叉反應(yīng) Human (predicted: Mouse,Rat)
產(chǎn)品應(yīng)用 WB=1:1000-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 90 kDa
檢測分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human STAT5b protein, around 500-600aa 
亞    型
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq, Jul 2008]

Subunit:
Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1, NMI and SOCS7. Interacts (via SH2 domain) with INSR.

Subcellular Location:
Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation.

Post-translational modifications:
Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphoryation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway.

Similarity:
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.

SWISS:
P51692

Gene ID:
6777

Database links:

Entrez Gene: 6777 Human

Entrez Gene: 20851 Mouse

Entrez Gene: 25126 Rat

Omim: 604260 Human

SwissProt: P51692 Human

SwissProt: P42232 Mouse

SwissProt: P52632 Rat

Unigene: 595276 Human

Unigene: 34064 Mouse

Unigene: 54486 Rat



產(chǎn)品圖片
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: HeLa, K562, PC-12, A20 Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 90 kDa Observed MW: 90 kDa
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