產品編號 | bsm-52222R |
英文名稱 | Smad1 Recombinant Rabbit mAb |
中文名稱 | 細胞信號轉導分子Smad-1重組兔單抗 |
別 名 | Mothers against decapentaplegic homolog 1; BSP 1; BSP1; BSP-1; Dwarfin A; DwfA; hSMAD 1; hSMAD1; JV 41; JV4 1; JV4-1; JV41; MAD mothers against decapentaplegic homolog 1; Mad related protein 1; MADH 1; MADH1; Madr 1; MADR1; Mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; Sma and Mad related protein 1; SMAD 1; SMAD-1; SMAD family member 1; SMAD mothers against DPP homolog 1 Drosophila; Spinal muscular atrophy distal with upper limb predominance; TGF beta signaling protein 1; Transforming growth factor beta signaling protein 1; SMAD1_HUMAN; MAD homolog 1; Mothers against DPP homolog 1; Mad-related protein 1; SMAD family member 1; Smad1; Transforming growth factor-beta-signaling protein 1. |
研究領域 | 腫瘤 細胞生物 信號轉導 干細胞 細胞凋亡 生長因子和激素 |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | 2D9 |
交叉反應 | Human |
產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 52 kDa |
檢測分子量 | |
細胞定位 | 細胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Recombinant human Smad1 protein, around N-terminal 1-200aa |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]. Function: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Subunit: Interacts with HGS, NANOG and ZCCHC12 (By similarity). May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with SKOR1. Interacts (via MH2 domain) with LEMD3. Binding to LEMD3 results in at least a partial reduction of receptor-mediated phosphorylation. Forms a ternary complex with PSMB4 and OAZ1 before PSMB4 is incorporated into the 20S proteasome. Subcellular Location: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. Tissue Specificity: Ubiquitous. Highest expression seen in the heart and skeletal muscle. Post-translational modifications: Phosphorylated on serine by BMP type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. DISEASE: Defects in SMAD1 may be a cause of primary pulmonary hypertension (PPH1) [MIM:178600]. A rare disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial PPH1 is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs. Similarity: Belongs to the dwarfin/SMAD family. Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain. SWISS: Q15797 Gene ID: 4086 Database links: Entrez Gene: 4086 Human Entrez Gene: 17125 Mouse Omim: 601595 Human SwissProt: Q15797 Human SwissProt: P70340 Mouse Unigene: 604588 Human Unigene: 223717 Mouse Unigene: 10635 Rat |
產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Smad1 monoclonal antibody, unconjugated (bsm-52222R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smad1 Monoclonal Antibody, Unconjugated(bsm-52222R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smad1 Monoclonal Antibody, Unconjugated(bsm-52222R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smad1 Monoclonal Antibody, Unconjugated(bsm-52222R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smad1 Monoclonal Antibody, Unconjugated(bsm-52222R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
|
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
| 亚洲人午夜射精精品日韩 | 欧美做爰爽爽爽爽爽爽 | 绿帽人妻-ThePorn | 成人免费a片xxx喷 | 少妇高潮毛片免费观看A | 国产精品久久久久久久电影渣男 | 欧美一级婬片A片久久精品色达人 | 人妻少妇孑伦无码视频 | 久久蜜精品国产亚洲AV不卡 | 成人免费色情网站在线播放 | 亚洲熟妇AV一区二区三区软件 | 失禁H啪肉尿出来高H受 | 无码人妻精品秘 一区二区 免费在线观看黄色视频网站 | 国产高清无码视频 | 国产亚洲精品成人a v久久网站 | 国语露脸刺激高清AV | 要灬要灬再深点受不了混乱 | 成人免费无码婬片在线观看免费 | 国产高清无码免费视频 | 国产午夜激无码毛片久久直播软件 | 无码人妻丰满熟妇一区二区三区 | 黄色在线观看视频网站 | 国产精品无码人妻无码色情多人 | 老司机深夜免费福利 | 国产成人中文网站 | 波多野结衣乳喷高潮五分高潮 | 一级婬片A级试看26分钟 | а天堂中文最新一区二区三区 | 91熟妇女人妻69丰满少妇 | 91人人妻人人做人人爽京东 | 亚洲中文在线视频 | 在线观看高清无码 | 5级黄18以上免费观看 | 女生流白浆免费视频观看 | 久久久久久人妻精品一区百度网盘 | 特一级一性一交一视频 | 午夜成人理论片A片AAA图片 | 国产又粗又大视频 | 成人高潮AAA一级毛片 | 搡BBBB搡BBB搡视频一级看 |