| 產(chǎn)品編號(hào) | bsm-33241M |
| 英文名稱 | HSP22 Mouse mAb |
| 中文名稱 | 熱休克蛋白-22單克隆抗體 |
| 別 名 | CMT2L; CRYAC; DHMN 2; DHMN2; E2 induced gene 1 protein; E2IG1; H11; Heat shock 22kDa protein 8; Heat shock protein 22; Heat shock protein beta 8; Hereditary motor neuropathy distal; HMN 2; HMN2; HSB8; HSPB 8; HSPB8; Protein kinase H11; Small stress protein like protein HSP22; Spinal muscular atrophy distal adult autosomal dominant; alpha crystallin C chain; Charcot Marie Tooth disease axonal type 2L; Charcot Marie Tooth disease spinal. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | 4C3 |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human HSP22 Protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Hsp22 (HSPB8) is a 196-amino acid protein that contains a central portion homologous to a highly conserved HSP-alpha crystallin domain common to all the small heat shock protein (HSP20) family members. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. The highest abundance of Hsp22 is in skeletal muscle, heart, and placenta. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L). Function: Displays temperature-dependent chaperone activity. Subunit: Monomer. Interacts with HSPB1. Interacts with DNAJB6. Subcellular Location: Cytoplasm. Nucleus. Note=Translocates to nuclear foci during heat shock. Tissue Specificity: Predominantly expressed in skeletal muscle and heart. DISEASE: Defects in HSPB8 are the cause of distal hereditary motor neuronopathy type 2A (HMN2A) [MIM:158590]; also known as distal hereditary motor neuropathy type IIA or spinal Charcot-Marie-Tooth disease IIA. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs. Defects in HSPB8 are the cause of Charcot-Marie-Tooth disease type 2L (CMT2L) [MIM:608673]. CMT2L is an axonal form of Charcot-Marie-Tooth disease. Axonal CMT neuropathies are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Similarity: Belongs to the small heat shock protein (HSP20) family. SWISS: Q9UJY1 Gene ID: 26353 Database links: Entrez Gene: 26353 Human Entrez Gene: 80888 Mouse Omim: 608014 Human SwissProt: Q9UJY1 Human SwissProt: Q9JK92 Mouse Unigene: 400095 Human Unigene: 21549 Mouse Unigene: 102906 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human 293T cell lysates
Lane 5: Human U-2 OS cell lysates
Primary: Anti-HSP22 (bsm-33241M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HSP22 Monoclonal Antibody, Unconjugated(bsm-33241M) at 1:200 overnight at 4°C, followed by conjugatio
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