| 產(chǎn)品編號 | bs-16410R |
| 英文名稱 | GTF2A1 alpha Rabbit pAb |
| 中文名稱 | 通用轉錄因子ⅡA亞基1/TFIIA-α抗體 |
| 別 名 | General transcription factor IIA subunit 1; General transcription factor IIA1; Glucose regulated protein 58kD pseudogene; gtf2a1; TF2A1; TF2AA_HUMAN; TFIIA 42; TFIIA alpha p55; TFIIA alpha p55, isoform 1; TFIIA p35 subunit; TFIIA-42; TFIIAL; Transcription initiation factor IIA alpha chain; Transcription initiation factor IIA subunit 1; Transcription initiation factor TFIIA 42 kDa subunit. |
| 研究領域 | 細胞生物 轉錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Sheep) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35/42 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GTF2A1 alpha: 1-100/376 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010] Function: TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. Subcellular Location: Nucleus. Post-translational modifications: The alpha and beta subunits are postranslationally produced from the precursor form by TASP1. The cleavage promotes proteasomal degradation. Similarity: Belongs to the TFIIA subunit 1 family. SWISS: P52655 Gene ID: 2957 Database links: Entrez Gene: 2957 Human Entrez Gene: 83602 Mouse Omim: 600520 Human SwissProt: P52655 Human SwissProt: Q99PM3 Mouse Unigene: 592334 Human Unigene: 275728 Mouse Unigene: 432242 Mouse Unigene: 44436 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
