| 產(chǎn)品編號 | bs-10090R |
| 英文名稱 | BMP6 Rabbit pAb |
| 中文名稱 | 骨形態(tài)發(fā)生蛋白6抗體 |
| 別 名 | Bone Morphogenic Protein 6; Decapentaplegic vegetal related; DVR6; BMP 6; BMP-6; TGFB related vegetal related growth factor; Transforming growth factor beta; Vegetal related(TGFB related) cytokine; Vg related sequence; VGR; VGR1; BMP6_HUMAN. |
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Specific References (7) | bs-10090R has been referenced in 7 publications.
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| 研究領(lǐng)域 | 細胞生物 生長因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 16/56 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BMP6: 421-513/513 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates a wide range of biological processes including iron homeostasis, fat and bone development, and ovulation. Differential expression of this gene may be associated with progression of breast and prostate cancer. Mutations in this gene may be associated with iron overload in human patients. [provided by RefSeq, Jul 2016] Function: Induces cartilage and bone formation. Subunit: Interacts with SOSTDC1. Subcellular Location: Secreted. Tissue Specificity: Expressed in the lung. Low levels seen in the kidney. Similarity: Belongs to the TGF-beta family. SWISS: P22004 Gene ID: 654 Database links: Entrez Gene: 654 Human Entrez Gene: 12161 Mouse Omim: 112266 Human SwissProt: P22004 Human SwissProt: P20722 Mouse Unigene: 285671 Human Unigene: 385759 Mouse Unigene: 40476 Rat |
| 產(chǎn)品圖片 |
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-BMP6 (bs-10090R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 16/56 kD
Observed band size: 76 kD
Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BMP6 Polyclonal Antibody, Unconjugated(bs-10090R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
