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Phospho1 Rabbit pAb (bs-12562R)  
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產(chǎn)品編號 bs-12562R
英文名稱 Phospho1 Rabbit pAb
中文名稱 細胞質(zhì)磷酸酶1抗體
別    名 EC 3.1.3.75; PHOP1_HUMAN; Phosphatase orphan 1; Phosphatase, orphan 1; phospho 1; phosphoethanolamine/phosphocholine phosphatase.  
研究領(lǐng)域 細胞生物  發(fā)育生物學(xué)  信號轉(zhuǎn)導(dǎo)  干細胞  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 30 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Phospho1: 201-267/267 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 PHOSPHO1 is a 267 amino acid phosphatase that is a member of the haloacid dehalogenase (HAD) superfamily of magnesium-dependent hydrolases. PHOSPHO1 is highly expressed in bone and cartilage and localizes to the osteoid layer of the periosteum. PHOSPHO1 is restricted to sites of mineralization and its inhibition decreases the ability of matrix vesicles to calcify in bone, suggesting that the protein may play a role in the matrix mineralization process during skeletal development. PHOSPHO1 cleaves phosphoethanolamine and phosphocholine to generate inorganic phosphate for bone mineralization. PHOSPHO1 contains three catalytic motifs that are conserved within the haloacid dehalogenase superfamily.

Function:
Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.

Tissue Specificity:
Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.

Similarity:
Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.

SWISS:
Q8TCT1

Gene ID:
162466

Database links:

Entrez Gene: 162466 Human

Entrez Gene: 237928 Mouse

Entrez Gene: 287644 Rat

SwissProt: Q8TCT1 Human

SwissProt: Q8R2H9 Mouse

SwissProt: B5DFB9 Rat



產(chǎn)品圖片
Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-Phospho1 (bs-12562R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 30 kD Observed band size: 30 kD
Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti- Phospho1 (bs-12562R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 35 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho1) Polyclonal Antibody, Unconjugated (bs-12562R ) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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