| 產(chǎn)品編號 | bs-7834R |
| 英文名稱 | CDCA8 Rabbit pAb |
| 中文名稱 | 細胞分裂周期蛋白8抗體 |
| 別 名 | BOR; BOREA_HUMAN; Borealin; CDCA8; Cell division cycle associated 8; Cell division cycle-associated protein 8; Dasra B; Dasra-B; DasraB; FLJ10468; FLJ12042; hDasra-B; MESRGP; OTTHUMP00000004514; Pluripotent embryonic stem cell-related gene 3 protein. |
| 研究領(lǐng)域 | 細胞生物 細胞周期蛋白 細胞分化 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Cow,Bee,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Borealin/CDCA8: 20-120/280 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Function: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Subunit: May form homooligomers and homodimers. Subcellular Location: Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere. Post-translational modifications: Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230. Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3. Similarity: Belongs to the borealin family. SWISS: Q53HL2 Gene ID: 55143 Database links: Entrez Gene: 55143 Human Entrez Gene: 52276 Mouse Omim: 609977 Human SwissProt: Q53HL2 Human SwissProt: Q8BHX3 Mouse Unigene: 524571 Human Unigene: 28038 Mouse Unigene: 14970 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Borealin/CDCA8 Polyclonal Antibody, Unconjugated(bs-7834R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
