| 產(chǎn)品編號 | bs-7541R |
| 英文名稱 | EDG3 Rabbit pAb |
| 中文名稱 | 內(nèi)皮分化型G蛋白偶聯(lián)受體3抗體 |
| 別 名 | EDG 3; EDG3; Endothelial differentiation G protein coupled receptor 3; Endothelial differentiation G-protein coupled receptor 3; Endothelial differentiation gene 3; Endothelial differentiation sphingolipid G protein coupled receptor 3; FLJ37523; FLJ93220; G protein coupled receptor endothelial differentiation gene 3; LPB 3; LPB3; S1P receptor 3; S1P receptor Edg 3; S1P receptor Edg-3; S1P receptor EDG3; S1P3; S1PR3; S1PR3_HUMAN; Sphingosine 1 phosphate receptor 3; Sphingosine 1 phosphate receptor Edg 3; Sphingosine 1 phosphate receptor Edg3; Sphingosine 1-phosphate receptor 3; Sphingosine 1-phosphate receptor Edg-3. |
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Specific References (5) | bs-7541R has been referenced in 5 publications.
[IF=6.117] Arianna Mazzoli. et al. SKELETAL MUSCLE INSULIN RESISTANCE AND ADIPOSE TISSUE HYPERTROPHY PERSIST BEYOND THE RESHAPING OF GUT MICROBIOTA IN YOUNG RATS FED A FRUCTOSE-RICH DIET. J NUTR BIOCHEM. 2022 Dec;:109247 WB ; Rat.
[IF=4.074] Dingkang Xu. et al. Sphingosine‐1‐phosphate receptor 3 is implicated in BBB injury via the CCL2‐CCR2 axis following acute intracerebral hemorrhage. 2021 Feb 28 WB,IF,IHC ; Human.
[IF=3.612] Hai B. Tran. et al. Dysregulated zinc and sphingosine-1-phosphate signaling in pulmonary hypertension: Potential effects by targeting of bone morphogenetic protein receptor type 2 in pulmonary microvessels. 2021 Aug 15 IF ; Rat.
[IF=3.388] Xiao et al. Fingolimod Suppresses a Cascade of Core Vicious Cycle in Dry Eye NOD Mouse Model: Involvement of Sphingosine-1-Phosphate Receptors in Infiltrating Leukocytes. (2017) Invest.Ophthalmol.Vis.Sci. 58:6123-6132 IHC ; Mouse.
[IF=2.733] Namiko et al. Up-regulation of Sphingosine-1-phosphate Receptors and Sphingosine Kinase 1 in the Peri-ischemic Area after Transient Middle Cerebral Artery Occlusion in Mice. Brain Res
. 2020 Jul 15;1739:146831. IHC ; mouse.
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| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2u/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EDG3: 145-250/378 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function. [provided by RefSeq, Jul 2008]. Function: Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q99500 Gene ID: 1903 Database links: Entrez Gene: 1903 Human Entrez Gene: 13610 Mouse Omim: 601965 Human SwissProt: Q99500 Human SwissProt: Q9Z0U9 Mouse Unigene: 585118 Human Unigene: 136736 Mouse Unigene: 23918 Rat |
| 產(chǎn)品圖片 |
Sample: Lane1: Heart (Mouse) Lysate at 40 ug Primary: Anti-EDG3 (bs-7541R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 42 kD Observed band size: 46 kD
Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Heart (Rat) Lysate at 40 ug
Lane 4: Heart (Mouse) Lysate at 40 ug
Primary: Anti-EDG3 (bs-7541R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-EDG3 antibody (bs-7541R)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
Th
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