產(chǎn)品編號 | bs-6801R |
英文名稱 | TCERG1 Rabbit pAb |
中文名稱 | 轉(zhuǎn)錄延伸調(diào)節(jié)蛋白1抗體 |
別 名 | CA 150; Co activator of 150 kDa; Coactivator of 150 kDa; MGC133200; TAF 2S; TAF2S; TATA box binding protein(TBP) associated factor RNA polymerase II S 150kD; TATA box binding protein associated factor 2S; TATA box binding protein associated factor RNA polymerase II S 150kD; TCERG 1; TCERG1; Transcription elongation regulator 1; Transcription factor CA 150; Transcription factor CA150; TCRG1_HUMAN. |
研究領(lǐng)域 | 腫瘤 轉(zhuǎn)錄調(diào)節(jié)因子 腫瘤細胞生物標志物 表觀遺傳學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 124 kDa |
檢測分子量 | |
細胞定位 | 細胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human TCERG1/CA150: 701-800/1098 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
CA150 is a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. It interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Function: Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter. Subunit: Binds formin (By similarity). Binds RNA polymerase II, HD and SF1. Subcellular Location: Nucleus. Tissue Specificity: Detected in brain neurons. Similarity: Contains 6 FF domains. SWISS: O14776 Gene ID: 10915 Database links: Entrez Gene: 10915 Human Entrez Gene: 56070 Mouse Omim: 605409 Human SwissProt: O14776 Human SwissProt: Q8CGF7 Mouse Unigene: 443465 Human Unigene: 723884 Human Unigene: 270511 Mouse |
產(chǎn)品圖片 |
Sample:
Testis(Mouse) Lysate at 40 ug
Primary: Anti-TCERG1 (bs-6801R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124kD
|
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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