| 產(chǎn)品編號 | bs-5601R |
| 英文名稱 | phospho-TPH (Ser260) Rabbit pAb |
| 中文名稱 | 磷酸化色氨酸羥化酶抗體 |
| 別 名 | Tph1(phospho S260); TPH(phospho Ser260); p-TPH(Ser260); MGC119994; TPH 1; TPH; TPH1; TPRH; Tryptophan 5 hydroxylase 1; Tryptophan 5 monooxygenase 1; Tryptophan 5 monooxygenase; Tryptophan hydroxylase 1; Tryptophan 5-hydroxylase 1; TPH1_HUMAN. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 免疫學 神經(jīng)生物學 信號轉(zhuǎn)導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human TPH around the phosphorylation site of Ser260: HS(p-S)DP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009]. Subunit: Homotetramer (By similarity). Tissue Specificity: Isoform 2 seems to be less widely expressed than isoform 1. Similarity: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain. SWISS: P17752 Gene ID: 7166 Database links: Entrez Gene: 7166 Human Entrez Gene: 21990 Mouse Omim: 191060 Human SwissProt: P17752 Human SwissProt: P17532 Mouse Unigene: 591999 Human Unigene: 248684 Mouse Unigene: 94788 Rat 色氨酸在腦中首先由色氨酸羥化酶(tryoptophan hydroxylase)催化生成5羥色氨酸(5hydroxytryptophan)，再經(jīng)脫羧酶作用生成5羥色胺，色氨酸羥化酶（TPH）是5-HT生物合成的限速酶，因此，色氨酸羥化酶的缺少可導致5-羥色胺的含量下降。色氨酸羥化酶主要在肝臟內(nèi)代謝。 |
| 產(chǎn)品圖片 |
Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-TPH (Ser260) (bs-5601R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Rat Cerebellum tissue lysates
Primary: Anti-phospho-TPH (Ser260) (bs-5601R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TPH (Ser260)) Polyclonal Antibody, Unconjugated (bs-5601R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
