| 產(chǎn)品編號 | bs-5486R |
| 英文名稱 | phospho-ERK5 ( Ser731+Thr733) Rabbit pAb |
| 中文名稱 | 磷酸化細胞外信號調(diào)節(jié)激酶5抗體 |
| 別 名 | ERK5(phospho Ser731+Thr733); Big MAP kinase 1; BMK 1; BMK 1 kinase; BMK-1; BMK1; BMK1 Kinase; EC 2.7.11.24; ERK 4; ERK 5; ERK-5; ERK4; Extracellular Signal Regulated Kinase 5; Extracellular signal-regulated kinase 5; MAP kinase 7; MAPK 7; MAPK7; Mitogen A |
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Specific References (2) | bs-5486R has been referenced in 2 publications.
[IF=7.59] Muzhe Li. et al. STS load PCL- MECM based hydrogel hybrid scaffold promote meniscal regeneration via modulating macrophage phenotype polarization. BIOMATER SCI-UK. 2023 Jan;: WB ; Rabbit.
[IF=7.36] Ye?F et al. Whole exome and target sequencing identifies MAP2K5 as novel susceptibility gene for familial non-medullary thyroid carcinoma. Int J Cancer. 2019 Mar 15;144(6):1321-1330. ICF&WB ; Human.
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| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領域 | 腫瘤 免疫學 信號轉(zhuǎn)導 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Cow,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 90 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human ERK5 around the phosphorylation site of Ser731+Thr733: VF(p-S)G(p-T)PK |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008] Function: Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Subunit: Interacts with MAP2K5. Forms oligomers. Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D. Interacts with SGK1. Subcellular Location: Cytoplasm. Nucleus. Note=Translocates to the nucleus upon activation. Tissue Specificity: Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver. Post-translational modifications: Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme. Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent. Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain. SWISS: Q13164 Gene ID: 5598 Database links: Entrez Gene: 5598 Human Entrez Gene: 23939 Mouse Omim: 602521 Human SwissProt: Q13164 Human SwissProt: Q9WVS8 Mouse Unigene: 150136 Human Unigene: 38172 Mouse Unigene: 144629 Rat
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| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Rat Muscle tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Primary: Anti-phospho-ERK5 ( Ser731+Thr733) (bs-5486R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 59 kDa
Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-ERK5 ( Ser731+Thr733)) Polyclonal Antibody, Unconjugated (bs-5486R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-ERK5 ( Ser731+Thr733)) Polyclonal Antibody, Unconjugated (bs-5486R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ERK5 ( Ser731+Thr733)) Polyclonal Antibody, Unconjugated (bs-5486R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-phospho-ERK5(Ser731+Thr733) Polyclonal Antibody, FITC conjugated (bs-5486R-FITC) 1:200, used at 1:200 dilution for 40 minutes at 37∑C.
Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-phospho-ERK5(Ser731+Thr733) Polyclonal Antibody, Unconjugated(bs-5486R) 1:200, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
