| 產品編號 | bs-3920R |
| 英文名稱 | ADCY2 Rabbit pAb |
| 中文名稱 | 腺苷酸環(huán)化酶2抗體 |
| 別 名 | adenylate cyclase 2; HBAC2; KIAA1060; type II adenylate cyclase; ADCY2_HUMAN. |
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Specific References (1) | bs-3920R has been referenced in 1 publications.
[IF=5.108] Yang Z et al. A?pilot?study?on?polycystic?ovarian?syndrome?caused?by?neonatal?exposure?to?tributyltin?and?bisphenol?A in?rats. Chemosphere.?2019 Sep;231:151-160. WB ; Rat.
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| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 生長因子和激素 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 124 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADCY2: 901-1091/1091 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
ADCY2 is a membrane-bound, calmodulin-insensitive adenylyl cyclase. Function: This is a membrane-bound, calmodulin-insensitive adenylyl cyclase. Subunit: Interacts with RAF1. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in brain. Post-translational modifications: Phosphorylated by RAF1. Similarity: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 guanylate cyclase domains. SWISS: Q08462 Gene ID: 108 Database links: Entrez Gene: 108 Human Entrez Gene: 210044 Mouse Omim: 103071 Human SwissProt: Q08462 Human SwissProt: Q80TL1 Mouse Unigene: 10731 Rat |
| 產品圖片 |
Sample: BV2 Cell (Mouse) Lysate at 40 ug
Primary: Anti- ADCY2 (bs-3920R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADCY2 Polyclonal Antibody, Unconjugated(bs-3920R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
