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ATG1/ULK1 Rabbit pAb (bs-3602R)  
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產(chǎn)品編號 bs-3602R
英文名稱 ATG1/ULK1 Rabbit pAb
中文名稱 自噬相關(guān)蛋白1抗體
別    名 ATG 1; ULK1; ATG1; ATG1A; Serine/threonine protein kinase ULK1; Serine/threonine protein kinase Unc51.1; ULK 1; Unc 51(C. elegans) like kinase 1; UNC 51; Unc 51 like kinase 1; Unc-51-like kinase 1; Unc-51 like kinase 1(C. elegans); UNC51; ULK1_HUMAN; Seri  
Specific References  (10)     |     bs-3602R has been referenced in 10 publications.
[IF=12.063] Jia Song. et al. Mesenchymal stromal cells ameliorate diabetes-induced muscle atrophy through exosomes by enhancing AMPK/ULK1-mediated autophagy. J CACHEXIA SARCOPENI. 2023 Jan;:  WB ;  Mouse.  
[IF=8.886] Bolin Cai. et al. LncEDCH1 improves mitochondrial function to reduce muscle atrophy by interacting with sarcoplasmic/endoplasmic reticulum calcium ATPase 2. Mol Ther-Nucl Acids. 2021 Dec;:  WB ;  Chicken.  
[IF=8.469] Cai, Bolin. et al. Long noncoding RNA ZFP36L2-AS functions as a metabolic modulator to regulate muscle development. CELL DEATH DIS. 2022 Apr;13(4):1-12  WB ;  Chicken.  
[IF=7.525] Ma Manting. et al. LncRNA-TBP mediates TATA-binding protein recruitment to regulate myogenesis and induce slow-twitch myofibers. CELL COMMUN SIGNAL. 2023 Dec;21(1):1-16  WB ;  Chicken.  
[IF=4.609] Shen et al. Cortical spreading depression preconditioning mediates neuroprotection against ischemic stroke by inducing AMP-activated protein kinase-dependent autophagy in a rat cerebral ischemic/reperfusion injury model. (2017) J.Neurochem. 140:799-813  WB ;  Rat.  
[IF=3.84] Shen, P. P., et al. "Cortical Spreading Depression (CSD) Preconditioning Mediates Neuroprotection against Ischemic Stroke by Inducing AMP‐activated Protein Kinase‐dependent Autophagy in a Rat Cerebral Ischemic/reperfusion Injury Model." Journal of Neurochemistry (2016).  WB ;  Rat.  
[IF=3.414] Ma HX et al. Mu-Xiang-You-Fang protects PC12 cells against OGD/R-induced autophagy via the AMPK/mTOR signaling pathway. J Ethnopharmacol. 2020 Jan 22;252:112583.  WB ;  Rattus norvegicus.  
[IF=3.322] Chunli Yang. et al. BMAL1 involved in autophagy and injury of thoracic aortic endothelial cells of rats induced by intermittent heat stress through the AMPK/mTOR/ULK1 pathway. BIOCHEM BIOPH RES CO. 2023 Jun;661:34  WB ;  Rat.  
[IF=2.97] Gao, Yuan, et al. "Activation of lysosomal degradative pathway in spinal cord tissues of carbon disulfide-treated rats." Chemico-Biological Interactions (2014).  WB ;  Rat.  
[IF=2.66] Song, Fuyong, et al. "Involvement of autophagy in tri-ortho-cresyl phosphate-induced delayed neuropathy in hens." Neurochemistry International (2013).  WB ;  
研究領(lǐng)域 腫瘤  細胞生物  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  細胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.5μg /Test,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 116 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ATG1/ULK1: 951-1050/1050 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 ULK1 belongs to the serine/threonine protein kinase family. It is involved in axon growth and plays an essential role in neurite branching during sensory axon outgrowth. Knockdown of ULK1 results in impaired endocytosis of nerve growth factor (NGF), excessive axon arborization, and severely stunted axon elongation indicating that ULK1 mediates a non clathrin coated endocytosis in sensory growth cones. Knockdown of ULK1 also inhibits the autophagic response. It appears to act as a convergence point for multiple signals that regulate autophagy, and in turn interacts with a large number of autophagy related (Atg) proteins.

Function:
Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

Subunit:
Interacts with GABARAP and GABARAPL2. Interacts (via C-terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation.

Subcellular Location:
Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.

Tissue Specificity:
Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Post-translational modifications:
Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
Contains 1 protein kinase domain.

SWISS:
O75385

Gene ID:
8408

Database links:

Entrez Gene: 8408 Human

Entrez Gene: 22241 Mouse

Entrez Gene: 360827 Rat

Omim: 603168 Human

SwissProt: O75385 Human

SwissProt: O70405 Mouse

Unigene: 47061 Human

Unigene: 271898 Mouse

Unigene: 24509 Rat



Atg1是一種絲氨酸/蘇氨酸蛋白激酶。Atg1的激脢活性是CVT信號傳導(dǎo)通路以及細胞自噬所必須的。Atg1可以和一些執(zhí)行細胞自噬的蛋白相互作用,而且有很多調(diào)控細胞自噬的信號傳導(dǎo)通路匯集在Atg1。因此Atg1可能是一個可以調(diào)控細胞自噬很多步驟的一個調(diào)節(jié)關(guān)鍵點。但在較高等的真核生物中,Atg1的角色仍然不是很清楚。通過目前的研究已經(jīng)比較了解細胞自噬可以導(dǎo)致細胞的死亡,但是如何導(dǎo)致死亡的分子機制還不清楚,有待于進一步研究。
產(chǎn)品圖片
Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-ATG1/ULK1 (bs-3602R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 116 kD Observed band size: 116 kD
Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATG1/ULK1 Polyclonal Antibody, Unconjugated(bs-3602R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ATG1/ULK1) polyclonal Antibody, Unconjugated (bs-3602R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Positive control: RSC96 Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody catalog number: bs-3602R; Dilution: 0.5μg in 100 μl 1X PBS containing 0.5% BSA
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