| 產(chǎn)品編號(hào) | bs-3437R |
| 英文名稱(chēng) | Phospho-Tyk2 (Tyr1054 + Tyr1055) Rabbit pAb |
| 中文名稱(chēng) | 磷酸化非受體酪氨酸蛋白激酶2抗體 |
| 別 名 | TYK2(phospho Y1054+Y1055); TYK2(phospho-Tyr1054/1055); Tyk2(phospho Y1054/1055); TYK2(phospho Tyr1054/1055); p-TYK2(Tyr1054/1055); JTK 1; JTK1; TYK 2; Non receptor tyrosine protein kinase 2; Non receptor tyrosine protein kinase TYK2; Protein Tyrosine Kinase 2; Tyrosine kinase 2; TYK2_HUMAN; Non-receptor tyrosine-protein kinase TYK2. |
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Specific References (3) | bs-3437R has been referenced in 3 publications.
[IF=9.756] Xueshuo Sun. et al. HDAC4 mediated LHPP deacetylation enhances its destabilization and promotes the proliferation and metastasis of nasopharyngeal carcinoma. CANCER LETT. 2023 May;562:216158 IHC ; Mouse,Human.
[IF=6.304] Chen Y et al. Dendritic cells-derived interferon-λ1 ameliorated inflammatory bone destruction through inhibiting osteoclastogenesis. Cell Death Dis. 2020 Jun 2;11(6):414. WB ; Mouse.
[IF=1.973] Liang Shao. et al. Betulonic acid regulates oviduct epithelial cell inflammation through the TLR4, MAPK, and JAK/STAT signalling pathways. REPROD FERT DEVELOP. 2023 May;35(8):480-491 WB ; Rat.
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| 產(chǎn)品類(lèi)型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 134 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Tyk2 around the phosphorylation site of Tyr1054/1055: HE(p-Y)(p-Y)RV |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the tyrosine kinase and, more specifically, the Janus kinases (JAKs) protein families. This protein associates with the cytoplasmic domain of type I and type II cytokine receptors and promulgate cytokine signals by phosphorylating receptor subunits. It is also component of both the type I and type III interferon signaling pathways. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with hyperimmunoglobulin E syndrome (HIES) - a primary immunodeficiency characterized by elevated serum immunoglobulin E. [provided by RefSeq]. Function: Probably involved in intracellular signal transduction by being involved in the initiation of type I IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain. Subunit: Interacts with JAKMIP1. Tissue Specificity: Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines. DISEASE: Defects in TYK2 are the cause of protein-tyrosine kinase 2 deficiency (TYK2 deficiency) [MIM:611521]; also known as autosomal recessive hyper-IgE syndrome (HIES) with atypical mycobacteriosis. TYK2 deficiency consists of a primary immunodeficiency characterized by recurrent skin abscesses, pneumonia, and highly elevated serum IgE. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. Contains 1 FERM domain. Contains 2 protein kinase domains. Contains 1 SH2 domain. SWISS: P29597 Gene ID: 7297 Database links: Entrez Gene: 7297 Human Entrez Gene: 54721 Mouse Omim: 176941 Human SwissProt: P29597 Human SwissProt: Q9R117 Mouse Unigene: 75516 Human Unigene: 20249 Mouse |
| 產(chǎn)品圖片 |
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-Tyk2(Tyr1054 + Tyr1055) (bs-3437R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 134 kD
Observed band size: 134 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Tyk2 (Tyr1054 + Tyr1055)) Polyclonal Antibody, Unconjugated (bs-3437R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Phospho-Tyk2(Tyr1054 + Tyr1055)) Polyclonal Antibody, Unconjugated (bs-3437R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Phospho-Tyk2(Tyr1054 + Tyr1055)) Polyclonal Antibody, Unconjugated (bs-3437R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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