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CD80 Rabbit pAb (bs-2211R)

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產(chǎn)品編號(hào)	bs-2211R
英文名稱	CD80 Rabbit pAb
中文名稱	刺激分子B7-1蛋白抗體
別名	Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80; CD80 antigen(CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80; CD80_RAT.
	Specific References (27) \| bs-2211R has been referenced in 27 publications. [IF=17.521] Yanlun Zhu. et al. Oral Delivery of Bioactive Glass-Loaded Core–Shell Hydrogel Microspheres for Effective Treatment of Inflammatory Bowel Disease. Advanced Science. 2023 Apr;:2207418 IHC ; Mouse. PubMed:37092589 [IF=10.761] Gaolong Lin. et al. Polydopamine-cladded montmorillonite micro-sheets as therapeutic platform repair the gut mucosal barrier of murine colitis through inhibiting oxidative stress. MATER TODAY BIO. 2023 Jun;20:100654 IF ; Mouse. PubMed:37214550 [IF=10.652] Lifen Wang. et al. Thermo-sensitive hydrogel with mussel-inspired adhesion enhanced the non-fibrotic repair effect of EGF on colonic mucosa barrier of TNBS-induced ulcerative colitis rats through macrophage polarizing. Chem Eng J. 2021 Jul;416:129221 IF ; Rat. PubMed:10.1016/j.cej.2021.129221 [IF=10.435] Xu, Hua-Zhen. et al. Synergy of nanodiamond–doxorubicin conjugates and PD-L1 blockade effectively turns tumor-associated macrophages against tumor cells. J Nanobiotechnol. 2021 Dec;19(1):1-24 IHC ; mouse. PubMed:34488792 [IF=8.806] Li TF et al. Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma.Biomaterials. 2018 Oct;181:35-52. IHC-P ; Human. PubMed:30071380 [IF=7.727] Jinbo Li. et al. Low dose shikonin and anthracyclines coloaded liposomes induce robust immunogenetic cell death for synergistic chemo-immunotherapy. J Control Release. 2021 Jul;335:306 IHC ; Mouse. PubMed:34081995 [IF=7.182] Chengxiao Wang. et al. Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation. Carbohyd Polym. 2021 May;:118211 IHC ; Mouse. PubMed:34127215 [IF=6.27] Bai J et al. Reactive Oxygen Species‐Scavenging Scaffold with Rapamycin for Treatment of Intervertebral Disk Degeneration. Adv Healthc Mater. 2019 Dec 10:e1901186. IHC-P,IHF-P&WB ; Rat&Mouse. PubMed:31820852 [IF=6.1] Xing Chang. et al. Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT. BIOCHEM PHARMACOL. 2023 May;:115582 IHC ; Human. PubMed:37146833 [IF=6.081] Xuan Wang. et al. Probiotic Bifidobacterium bifidum G9-1 Has a Preventive Effect on the Acceleration of Colonic Permeability and M1 Macrophage Population in Maternally Separated Rats. Biomedicines. 2021 Jun;9(6):641 IHC ; Rat. PubMed:34204993 [IF=5.345] Yuan SJ et al. Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.J Nanobiotechnology. 2019 Oct 17;17(1):110. IHC-P ; Mouse. PubMed:31623629 [IF=5.215] Tianrui Zhang. et al. Daphnetin Improves Neuropathic Pain by Inhibiting the Expression of Chemokines and Inflammatory Factors in the Spinal Cord and Interfering with Glial Cell Polarization. PHARMACEUTICALS-BASE. 2023 Feb;16(2):243 IF ; Rat. PubMed:10.3390/ph16020243 [IF=5.19] Han, Sheng, et al. "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 83. WB ; Human. PubMed:28641579 [IF=4.854] Linghao Xiao. et al. Osteogenic capacity of octacalcium phosphate involving macrophage polarization. J BIOMED MATER RES A. 2022 Dec;: IHC ; Rat. PubMed:36573692 [IF=3.701] Keiichi Hiramoto. et al. Innate immune activation and antitumor effects of Lactobacillus-fermented Sparassis crispa extract in mice. J Funct Foods. 2020 Dec;75:104215 IHC ; Mouse. PubMed:10.1016/j.jff.2020.104215 [IF=3.616] Moser PT et al. Creation of Laryngeal Grafts from Primary Human Cells and Decellularized Laryngeal Scaffolds. Tissue Eng Part A. 2019 Oct 30. IHC-P ; PubMed:31663421 [IF=3.496] Philipp T Moser et al. Creation of Laryngeal Grafts from Primary Human Cells and Decellularized Laryngeal Scaffolds. Tissue Eng Part A. 2020 May;26(9-10):543-555. IF ; Rat. PubMed:31663421 [IF=3.251] Masataka Kadowaki. et al. Involvement of M1/M2 Macrophage Polarization in Reparative Dentin Formation. LIFE-BASEL. 2022 Nov;12(11):1812 IF ; Human. PubMed:36362965 [IF=3.118] Yu S et al. Dendritic cells modified with Der p1 antigen as a therapeutic potential for allergic rhinitis in a murine model via regulatory effects on IL-4, IL-10 and IL-13.Int Immunopharmacol. 2019 May;70:216-224. ICF ; Mouse. PubMed:30851701 [IF=2.976] Peng?Y et al. Sonodynamic therapy improves anti?tumor immune effect by increasing the infiltration of CD8+ T cells and altering tumor blood vessels in murine B16F10 melanoma xenograft. Oncol Rep.?2018 Oct;40(4):2163-2170.? WB ; Mouse. PubMed:30106435 [IF=2.76] da Silva Junior, Evaldo Moreira, et al. "Modulating effect of low intensity pulsed ultrasound on the phenotype of inflammatory cells." Biomedicine & Pharmacotherapy (2017). IHC-P ; Rat. PubMed:29191696 [IF=2.744] Maria Apriliani Gani. et al. Bovine hydroxyapatite-based scaffold accelerated the inflammatory phase and bone growth in rats with bone defect. J APPL BIOMATER FUNC. ;(): IHC ; Rat. PubMed:36708249 [IF=2.674] Leiblein M et al. Size matters: Effect of granule size of the bone graft substitute (Herafill?) on bone healing using Masquelet's induced membrane in a critical size defect model in the rat's femur. J Biomed Mater Res B Appl Biomater. 2019 Nov 13. IHC-P ; Rat. PubMed:31721435 [IF=2.485] Ju et al. An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model. (2016) BMC.Immunol. 17:31 IHC ; Mouse. PubMed:27669687 [IF=2.46] Wang, Shan, et al. "5-Aminolevulinic Acid–mediated Sonodynamic Therapy Reverses Macrophage and Dendritic Cell Passivity in Murine Melanoma Xenografts." Ultrasound in Medicine & Biology (2014). IHC-P ; Mouse. PubMed:25023114 [IF=1.58] Liang, Zhuoping, et al. "Role of dendritic cells in consistency between upper and lower airway inflammatory responses: animal experimental studies." Int J Clin Exp Pathol 9.10 (2016): 10093-10104. IHC-P ; Rat. PubMed:ISSN:1936-2625/IJCEP0035434 [IF=1.55] Chen, Quan, et al. "Prevention of ultraviolet radiation?induced immunosuppression by sunscreen in Candida albicans?induced delayed?type hypersensitivity." Molecular Medicine Reports (2016). WB ; Mouse. PubMed:27175551
研究領(lǐng)域	腫瘤干細(xì)胞 b-淋巴細(xì)胞
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human,Mouse,Rat
產(chǎn)品應(yīng)用	WB=1:500-2000,Flow-Cyt=2μg/Test not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	30 kDa
檢測(cè)分子量	60-65
細(xì)胞定位	細(xì)胞膜
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from rat CD80: 251-321/321 <Cytoplasmic>
亞型	IgG
純化方法	affinity purified by Protein A
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng)	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. Function: Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed on activated B-cells, macrophages and dendritic cells. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Database links: Entrez Gene: 941 Human Entrez Gene: 12519 Mouse Omim: 112203 Human SwissProt: P33681 Human SwissProt: Q00609 Mouse Unigene: 838 Human Unigene: 89474 Mouse
產(chǎn)品圖片	Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug Lane 3: Thymus (Rat) Lysate at 40 ug Lane 4: Raji (Human) Cell Lysate at 30 ug Lane 5: K562 (Human) Cell Lysate at 30 ug Primary: Anti-CD80 (bs-2211R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60-65 kD Observed band size: 60 kD Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug Lane 3: Thymus (Rat) Lysate at 40 ug Lane 4: Raji (Human) Cell Lysate at 30 ug Lane 5: K562 (Human) Cell Lysate at 30 ug Primary: Anti-CD80 (bs-1479R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60-65 kD Observed band size: 60 kD

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標(biāo)記二抗

	1、抗體溶解方法
	2、抗體修復(fù)方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關(guān)于肽鏈的設(shè)計(jì)
	9、多肽的溶解與保存
	10、酶標(biāo)抗體效價(jià)測(cè)定程序

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