| 產(chǎn)品編號(hào) | bs-0677R |
| 英文名稱 | Angiopoietin 2 Rabbit pAb |
| 中文名稱 | 血管生成素2抗體 |
| 別 名 | AGPT 2; AGPT-2; Agpt2; ANG 2; ANG2; ANG-2; Angiopoietin 2a; Angiopoietin 2B; Angiopoietin2; Angiopoietin-2; ANGPT 2; ANGPT2; Tie2 ligand; ANGP2_HUMAN. |
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Specific References (6) | bs-0677R has been referenced in 6 publications.
[IF=6.68] Do HS et al. Enhanced thrombospondin-1 causes dysfunction of vascular endothelial cells derived from Fabry disease-induced pluripotent stem cells. EBioMedicine. 2020 Jan 22;52:102633. IHC-P ; Human.
[IF=3.149] Yuksel Olgun. et al. The Effect of Bevacizumab and Propranolol on Nasal Polyposis. INT J CLIN PRACT. 2022 Oct 12;2022:6174664 IHC ; Human.
[IF=3.072] Xue Y et al. miR‐205‐5p inhibits psoriasis‐associated proliferation and angiogenesis: Wnt/β‐catenin and mitogen‐activated protein kinase signaling pathway are involved. J Dermatol
. 2020 Aug;47(8):882-892. WB ; Mouse&Human.
[IF=2.84] Zhao, Qiuchen, et al. "Intranasal administration of Human umbilical cord mesenchymal stem cells-conditioned medium enhances vascular remodeling after stroke." Brain Research (2015). WB ; Rat.
[IF=2.14] Lv, Ming-ming, Xin-dong Fan, and Li-xin Su. "Is a Swine Model of Arteriovenous Malformation Suitable for Human Extracranial Arteriovenous Malformation? A Preliminary Study." Cardiovascular and interventional radiology (2013): 1-7. IHC-P ;
[IF=0] Du, Weiyan, et al. "The Expression and Significance of Angiopoietin-2 and Glucose Regulated Protein78 in Colorectal Cancer." Cancer Cell Research (2016). IHC-P ; Human.
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| 研究領(lǐng)域 | 腫瘤 心血管 生長(zhǎng)因子和激素 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Pig,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Angiopoietin 2: 401-496/496 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Subunit: Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. Subcellular Location: Secreted. Similarity: Contains 1 fibrinogen C-terminal domain. SWISS: O15123 Gene ID: 285 Database links: Entrez Gene: 285 Human Entrez Gene: 11601 Mouse Omim: 601922 Human SwissProt: O15123 Human SwissProt: O35608 Mouse Unigene: 583870 Human Unigene: 439874 Mouse Unigene: 138360 Rat 血管生成素2(angiopoietin-2, Ang-2)是Ang家族的成員之一,是特異性血管生成刺激因子,也是惡性腫瘤早期的標(biāo)志分子. |
| 產(chǎn)品圖片 |
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-Angiopoietin 2 (bs-0677R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Ang2 Polyclonal Antibody, Unconjugated(bs-0677R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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