| 產(chǎn)品編號 | bs-2034R |
| 英文名稱 | CRIM1 Rabbit pAb |
| 中文名稱 | 富含半胱氨酸運動神經(jīng)元蛋白1抗體 |
| 別 名 | Cysteine-rich motor neuron 1 protein; CRIM-1; Cysteine-rich repeat-containing protein S52; Processed cysteine-rich motor neuron 1protein; CRIM 1; CRIM1; Cysteine rich motor neuron 1 protein; Cysteine rich repeat containing protein S52; Cysteine rich transmembrane BMP regulator 1(chordin like); Cysteine rich transmembrane BMP regulator 1; MGC138194; Processed cysteine rich motor neuron 1 protein; S52; CRIM1_HUMAN. |
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Specific References (2) | bs-2034R has been referenced in 2 publications.
[IF=4.556] Diqi Yang. et al. Essential Role of CRIM1 on Endometrial Receptivity in Goat. Int J Mol Sci. 2021 Jan;22(10):5323 WB ; Goat.
[IF=3.39] Zeng, Hui, et al. "CRIM1, a newfound cancer-related player, regulates the adhesion and migration of lung cancer cells." Growth Factors (2016): 1-9. WB ; Human.
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| 研究領(lǐng)域 | 心血管 細(xì)胞生物 染色質(zhì)和核信號 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 血管內(nèi)皮細(xì)胞 細(xì)胞骨架 細(xì)胞外基質(zhì) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 110 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CRIM1: 901-1028/1028 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
CRIM1 interacts with BMP4 (bone morphogenic protein 4) and BMP7 and modulates BMP activity by affecting their processing and delivery to the cell surface. By interacting with growth factors implicated in motor neuron differentiation and survival, it may play a role in CNS development. It may also play a role in capillary formation and maintenance during angiogenesis. Function: May play a role in CNS development by interacting with growth factors implicated in motor neuron differentiation and survival. May play a role in capillary formation and maintenance during angiogenesis. Modulates BMP activity by affecting its processing and delivery to the cell surface. Subcellular Location: Secreted and Cell membrane. Tissue Specificity: Expressed in pancreas, kidney, skeletal muscle, lung, placenta, brain, heart, spleen, liver and small intestine. Expressed in blood vessels (at protein level). Post-translational modifications: N-glycosylated. Similarity: Contains 4 antistasin-like domains. Contains 1 IGFBP N-terminal domain. Contains 6 VWFC domains. SWISS: Q9JLL0 Gene ID: 50766 Database links: Entrez Gene: 51232 Human Entrez Gene: 50766 Mouse Omim: 606189 Human SwissProt: Q9NZV1 Human SwissProt: Q9JLL0 Mouse Unigene: 699247 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human Jurkat cell lysates
Primary: Anti-CRIM1 (bs-2034R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 140 kDa
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CRIM1) Polyclonal Antibody, Unconjugated (bs-2034R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CRIM1) Polyclonal Antibody, Unconjugated (bs-2034R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CRIM1) Polyclonal Antibody, Unconjugated (bs-2034R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CRIM1) Polyclonal Antibody, Unconjugated (bs-2034R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CRIM1) Polyclonal Antibody, Unconjugated (bs-2034R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CRIM1) Polyclonal Antibody, Unconjugated (bs-2034R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CRIM1) Polyclonal Antibody, Unconjugated (bs-2034R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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