| 產(chǎn)品編號(hào) | bs-1092R |
| 英文名稱 | MRP2/ABCC2 Rabbit pAb |
| 中文名稱 | 多藥耐藥相關(guān)蛋白2抗體 |
| 別 名 | multidrug resistance-associated protein2; ABC30; ABCC2; ATP binding cassette sub family C(CFTR/MRP) member 2; ATP binding cassette subfamily C member 2; Canalicular multidrug resistance protein; Canalicular multispecific organic anion transporter 1; CMOAT |
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Specific References (11) | bs-1092R has been referenced in 11 publications.
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| 研究領(lǐng)域 | 腫瘤 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 174 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MRP2: 485-615/1545 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
multidrug resistance-associated protein 2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia. Belongs to the ABC transporter family. Function: Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver. DISEASE: Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function. Similarity: Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains. SWISS: Q92887 Gene ID: 1244 Database links: Entrez Gene: 1244 Human Entrez Gene: 12780 Mouse Omim: 601107 Human SwissProt: Q92887 Human SwissProt: Q8VI47 Mouse Unigene: 368243 Human Unigene: 39054 Mouse Unigene: 10265 Rat
MRP-2作為一種結(jié)合輸出泵，轉(zhuǎn)運(yùn)許多不同的藥物結(jié)合物的蛋白。 MRP2蛋白的染色陽性產(chǎn)物分布于細(xì)胞漿/膜中。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MRP2/ABCC2 Polyclonal Antibody, Unconjugated (bs-1092R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
The A549(H) cells were fixed with 4% PFA (10 min at r.t.). Primary Antibody (green): Rabbit Anti-MRP2/ABCC2 antibody (bs-1092R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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