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PFKFB3/PFK2 Recombinant Rabbit mAb (bsm-61693R)  
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產(chǎn)品編號 bsm-61693R
英文名稱 PFKFB3/PFK2 Recombinant Rabbit mAb
中文名稱 果糖-2,6-二磷酸酶3/磷酸果糖激酶2重組兔單抗
別    名 6-bisphosphatase; 6-P2ase 3; Fructose-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; uPFK 2.  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 3F10
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:20-50,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 60 kDa
檢測分子量 60
性    狀 Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human PFKFB3 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
保存條件 Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Catalyzes both the synthesis and degradation of fructose 2,6-bisphosphate.

SWISS:
Q16875

Gene ID:
5209

IPFK2是糖酵解的一種重要酶,廣泛存在于各種生物細胞中。由于該酶對調(diào)控糖酵解通路的重要作用.
產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with PFKFB3/PFK2 monoclonal antibody, unconjugated (bsm-61693R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PFKFB3/PFK2 Monoclonal Antibody, Unconjugated(bsm-61693R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PFKFB3/PFK2 Monoclonal Antibody, Unconjugated(bsm-61693R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PFKFB3/PFK2 Monoclonal Antibody, Unconjugated(bsm-61693R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PFKFB3/PFK2 Monoclonal Antibody, Unconjugated(bsm-61693R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PFKFB3/PFK2 Monoclonal Antibody, Unconjugated(bsm-61693R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PFKFB3/PFK2 Monoclonal Antibody, Unconjugated(bsm-61693R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PFKFB3/PFK2 Monoclonal Antibody, Unconjugated(bsm-61693R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PFKFB3/PFK2 Monoclonal Antibody, Unconjugated(bsm-61693R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PFKFB3/PFK2 Monoclonal Antibody, Unconjugated(bsm-61693R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PFKFB3/PFK2 Monoclonal Antibody, Unconjugated(bsm-61693R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PFKFB3/PFK2 Monoclonal Antibody, Unconjugated(bsm-61693R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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