| 產品編號 | bsm-61674R |
| 英文名稱 | Phospho-PAK4/5/6 (Ser474/Ser602/Ser560) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化PAK4/5/6重組兔單抗 |
| 別 名 | PAK4; PAK5; PAK6. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:1000-1:2000,Flow-Cyt=1:20-1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 64,75,81 kDa |
| 檢測分子量 | 64,80,75 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PAK4 around the phosphorylation site of S474 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. SWISS: O96013 Gene ID: 10298 絲氨酸/蘇氨酸蛋白激酶4 (PAK4)是新發(fā)現的一類蛋白因子，在細胞信號轉導過程中起很重要的作用。Pak4常在人體的多種組織器官的腫瘤細胞中呈現過表達，他的絲氨酸位點很可能是Pak4酶活性區(qū)域中的自身磷酸化位點，絲氨酸到谷氨酸位點的突變體促使其獲得了酶活性。在腫瘤遺傳學、細胞癌變、細胞凋亡等一些重要的細胞生物學研究中，具有重要的意義。 |
| 產品圖片 |
Western blot analysis of U87-MG cell lysate. Using Phospho-PAK4/5/6 (Ser474/Ser602/Ser560) (bsm-61674R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
