| 產(chǎn)品編號 | bsm-61594R |
| 英文名稱 | BCHE Recombinant Rabbit mAb |
| 中文名稱 | 丁酰膽堿酯酶重組兔單抗 |
| 別 名 | BCHE; Butyrylcholine esterase; CHE1; CHE2; Choline esterase II; Cholinesterase(serum) 2; Cholinesterase 1; Cholinesterase; E1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 4B3 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68 kDa |
| 檢測分子量 | 90 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cholinesterase |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. SWISS: P06276 Gene ID: 590 血清丁酰膽堿酯酶是由四個亞基組成的糖蛋白，分子量28萬～35萬。作為一類糖蛋白，主要存在于腦、肝、血及膽堿能神經(jīng)末稍的突觸間隙中，主要由肝臟合成，并存在于血清中，生理作用不明，稱為假性膽堿酯酶或丁酰膽堿酯酶[1]Lockridge等采用Edman降解法測定了人血清BChE完整的氨基酸序列，共含有574個氨基酸殘基。BChE氨基酸序列與其他酯酶家族蛋白有很高的同源性。以電鰩AChE的三維結構為模板對人BChE的三維結構進行計算機模擬結果表明，BChE活性中心，囊袋的底部為Ser198，其兩側相距一定距離分別 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with BCHE monoclonal antibody, unconjugated (bsm-61594R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
