| 產(chǎn)品編號 | bsm-61546R |
| 英文名稱 | Prothrombin Recombinant Rabbit mAb |
| 中文名稱 | 凝血酶原重組兔單抗 |
| 別 名 | Coagulation factor II; F2; Factor II; Prepro coagulation factor II; Prothrombin; prothrombin B-chain; PT; RPRGL2; serine protease; THPH1; Thrombin heavy chain. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 70 kDa |
| 檢測分子量 | 70 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Prothrombin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. SWISS: P00734 Gene ID: 2147 正常肝細胞中，在內(nèi)質(zhì)網(wǎng)內(nèi)可將DCP羧化使之成為有活性的凝血酶原，一旦機體需要，凝血酶原即可轉(zhuǎn)變成凝血酶參與機體的各項生理功能，當有腫瘤細胞發(fā)生時，DCP明顯升高。DCP主要用于肝癌方面的研究；有學(xué)者認為：聯(lián)合應(yīng)用DCP和AFP對于早期診斷HCC（原發(fā)性肝細胞癌）有較大的幫助。 |
| 產(chǎn)品圖片 |
Western blot analysis of human serum lysate. Using F2 (bsm-61546R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
