| 產品編號 | bsm-61500R |
| 英文名稱 | CAPN1 Recombinant Rabbit mAb |
| 中文名稱 | 鈣蛋白酶1重組兔單抗 |
| 別 名 | Calpain-1 catalytic subunit; CANP; CANP small subunit; CANPL1; CAPN1; Micromolar Calpain; Mu Calpain; muCANP. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 82 kDa |
| 檢測分子量 | 75,80 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Calpain 1 catalytic subunit |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7). SWISS: P07384 Gene ID: 823 鈣蛋白酶(calpain)是一族鈣離子依賴性的、水解半胱氨酸的蛋白酶,廣泛存在于全身各臟器組織中。鈣蛋白酶參與不同細胞過程,包括細胞骨架和細胞膜附著的構型重建、不同的信號轉導途徑和細胞凋亡等. |
| 產品圖片 |
Western blot analysis of Human fetal kidney lysate. Using CAPN1 (bsm-61500R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed A431 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CAPN1) monoclonal Antibody, unconjugated (bsm-61500R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
