| 產(chǎn)品編號 | bsm-61260R |
| 英文名稱 | Phospho-HSL (Ser853) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化荷爾蒙敏感脂肪酶重組兔單抗 |
| 別 名 | Hormone sensitive lipase; LIPE; LIPS; HSL. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:1000-1:5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 117 kDa |
| 檢測分子量 | 83 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Hormone sensitive lipase around the phosphorylation site of S853 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. SWISS: Q6NSL7 Gene ID: 3991 HSL又稱：敏感性甘油三酯脂肪酶，在脂肪動員中，脂肪細胞內(nèi)激素敏感性甘油三酯脂肪酶（HSL）起決定性作用，它是脂肪分解的限速酶，稱為激素敏感性脂肪酶。腎上腺素、去甲腎上腺素、胰高血糖素等激活HSL促進脂肪動員；胰島素，前列腺素E及煙酸等抑制HSL，抑制脂肪的動員。分子量：116KDa。 |
| 產(chǎn)品圖片 |
Western blot analysis of (1) Mouse muscle lysate; (2) Mouse muscle lysate treated with AP. Using Phospho-HSL (Ser853) (bsm-61260R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
