| 產(chǎn)品編號 | bsm-63059R |
| 英文名稱 | phospho-BAD (S75) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化相關(guān)死亡促進因子重組兔單抗 |
| 別 名 | Bad (phospho S75); Bad (phospho Ser75); p-Bad (S75);p- Bad (Ser75); p-Bad (phospho Ser75); BBC 2; BBC2; BBC6; Bcl 2 Antagonist of Cell Death; Bcl 2 Binding Component 6; BCL X / BCL 2 Binding Protein; BCL X Binding Protein; Bcl XL/Bcl 2 Associated Death Promoter; Bcl-2-like protein 8; Bcl2 antagonist of cell death; BCL2 antagonist of cell death protein; BCL2 associated agonist of cell death; Bcl2 Associated Death Promoter; BCL2 binding component 6; BCL2 binding protein; Bcl2 Like 8 Protein; Bcl2-L-8; BCL2L8; BclXL; Proapoptotic BH3 Only Protein; BAD_HUMAN; Bcl-2-binding component 6. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 19D16 |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 18 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human BAD around the phosphorylation site of Ser75 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform. SWISS: Q92934 Gene ID: 572 BAD是BCL2/BAX、BCL-XL/BAX異二聚體的負調(diào)節(jié)基因。BAD是BCL2/BCL-XL相關(guān)死亡促進因子，作為BCL2、 bCL-XL異二聚體伴分子而促進細胞凋亡。 有學(xué)者認為：BAD缺乏典型的羧基端跨膜結(jié)構(gòu)，提示其并非一完整膜蛋白。與同BCL2作用相比，BAD與BCL-XL的結(jié)合更強，BAD以濃度依賴性方式替換BCL-XL/BAX、BCL2/BAX異二聚體中的BAX，使BAX游離而促進細胞凋亡。當一細胞系的所有細胞內(nèi)異二聚體（BCL-XL/BAX和BCL2/BAX）的含量≥50%時，則細胞耐 |
| 產(chǎn)品圖片 |
Hela (H) cells were treated with or without Calyculin A (50nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-BAD (S75) monoclonal antibody, unconjugated (bsm-63059R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
